Female mice 4 - 6 weeks old were intraperitoneally injected with 10iu of pregnant mares serum gonadotropin ( pmsg ) , and 48h later with 10iu human chorionic gonadotropin ( hcg ) 细胞周期进人m期以前, mpf的活性调节依赖cdc25和myt激酶之间的平衡,其平衡状态决定细胞周期进程。
Sodn oligodeoxynucleotide of pkb : 5cac atg gac gtc aaa gct tcc aa3 ' each base of both sodn and saodn oligodeoxynucleotide was sulfurated . methods 1 . superovulation , natural mating and eggs collection Myt激酶的作用与cdc25相反,可以持续的使edcz的nlr14和t ”巧处于磷酸化状态而抑制mpf的活性,从而抑制细胞周期进程。
Abtibidies goat anti - pkb polyclonal antibodies , antiphospho - tyr15 of cdc2 and antiphospho - ser216 of cdc25c polyclonal antibodies , alkaline phosphatase conjugated anti - goat secondary antibodies were purchased from santa cruz biotechnology 与edcz激酶增生细胞核抗原。一myt和c一myb基因互补的odn均可抑制平滑肌细胞的增生和移行。
Rt - pcr kit was from takara technology co . ltd . the sequence of the primer of pkb are shown below , together with all the sodn and saodn oligodeoxynucleotide were synthesized in takara technology co . ltd . pkb primers ; forward primer 5 ' cga gaa gcc gcg acc caa cac3 ' and reverse primer 5atg cac tac cgc cgg a3 " 有丝分裂前, cdcz / cy山b 、组蛋白h ;激酶的活性通过被weel和myt激酶磷酸化cdcz的1卜巧和thr14残基而抑制。在有丝分裂期mpf可以被cdc25活化,这两个位点被去磷酸化, cdc25是蛋白水解酶,可以使cdcz的t打巧脱离磷酸化。
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