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t vector in Chinese

Pronunciation:
How to pronounce "t vector"Use "t vector" in a sentence
TranslationMobile
  • t向量
Examples
  • Then the pcr product was purified , ligated into pgem - t vector by ta cloning
    筛选阳性克隆,测序,大量制备序列完全正确的质粒。
  • The pcr product was cloned into pmd18 - t vector . the positive recombinant clone was identified by pcr and endonuclease digest
    提取重组质粒经pcr鉴定和酶切鉴定后,对插入片段进行序列测定及分析。
  • The product of pcr named vp6 is approximate 1 . 3kb in length . the vp6 gene was cloned into pmd18 - t vector and sequenced
    将其插入克隆载体质粒pmd18 - t的ecorv酶切位点处,构建重组质粒pmd18 - t ? vp6 。
  • Pgem - t vector system , jm109 competent cells , reverse transcription kit and in vitro translation kit were purchased from promega company
    Pgem一t载体,感受态细菌jm109 ,反转录试剂盒和体外翻译试剂盒均购自promega公司。
  • 2 . pcr products can be purified by ctab method , which removing the dntp and primers . the purified products can be used in the ligation reaction to pmd18 - t vector with high efficiency
    2 、采用ctab进行pcr产物的纯化,以去除pcr产物中的引物和dntp ,所得产物可用于与t载体连接反应,以提高连接效率。
  • 2 . using a pair of degenerate primers based on the conserved region , hmg - box , of human sry gene , eight different fragments were amplified from both female and male rana rugulosa wiegmann then cloned by using pmd18 - t vector and sequenced
    2 、参照人sox基因设计了一对兼并引物,扩增了虎纹蛙的sox基因,并对扩增产物进行了克隆和测序。
  • To device a primer pairs and amplify the full length pstvd by rt - pcr , the positive rna extraction from tuber sample was used as template . the product of rt - pcr was purified and connected to the plasmid pmd 18 - t vector
    Cdna核酸斑点杂交反应( nash )检测pstvd方法准确、灵敏度高,一次检测样品数量多,且对于异地样品检测非常方便,是以往其它检测方法的有效补充。
  • Two degenerate primers were designed and synthesized according to the highly conservative sequences among the known - glc genes . a cdna fragment of 208bp was amplified by rt - pcr , which was subsequently cloned into pmd18 - t vector for sequencing analysis
    利用genbank中已经登录的其它植物中该酶基因的保守序列,设计一对简并引物,采用rt - pcr技术,从茶树扩增出208bp的cdna片段。
  • The tmek2 and tmek2mut was amplified by pcr and then cloned to pmd 18 - t vector . to express the genes in plants , the coding region of tmek2 and tmek2mut were placed between the 35s promoter and nos terminator of pibl , respectively
    载体构建的过程包括目的基因的pcr扩增、目的基因连接到pmd18 - t载体、目的基因连接到中间表达载体pib1 、目的基因及启动子、终止子连接到表达载体pbin19 。
  • Designing a specific primer pair based on the sequence of the gene ubia in e . coli mc4100 , a dna fragment was amplified from the genomic dna of e . coli mc4100 by pcr and then was cloned into pucm - t vector . the cloned fragment was sequenced
    根据e . colimc4100的ubia基因全碱基顺序,合理设计引物,以e . colimc4100基因组dna为模板进行pcr扩增,将pcr产物克隆于pucm - tvector后对其进行测序鉴定。
  • More examples:  1  2  3  4
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