×

anchored pcr meaning

  "anchored pcr" in a sentence
  • [Medicine]
    In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules,annealing of the primers to their complementary sequences,and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient,specific,and extremely sensitive. Uses for the reaction include disease diagnosis,detection of difficult-to-isolate pathogens,mutation analysis,genetic testing,DNA sequencing,and analyzing evolutionary relationships.

Examples

  1. RACE can provide the sequence of an RNA transcript from a small known sequence within the transcript to the 5'end ( 5'RACE-PCR ) or 3'end ( 3'RACE-PCR ) of the RNA . This technique is sometimes called " one-sided PCR " or " anchored PCR ".

Related Words

  1. anchorage system meaning
  2. anchorage zone meaning
  3. anchored bulkhead meaning
  4. anchored genomic island meaning
  5. anchored genomic islands meaning
  6. anchored polymerase chain reaction meaning
  7. anchored-type ceramic veneer meaning
  8. anchoress meaning
  9. anchoretic meaning
  10. anchoretical meaning
PC Version